Plasmid Preparation:Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development
Article Snippet: .. Plasmid Constructs Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 . .. A 29-mer non-effective oligonucleotide (Origene) cloned in the same vector was used as control.
Construct:Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development
Article Snippet: .. Plasmid Constructs Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 . .. A 29-mer non-effective oligonucleotide (Origene) cloned in the same vector was used as control.
shRNA:Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development
Article Snippet: .. Plasmid Constructs Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 . .. A 29-mer non-effective oligonucleotide (Origene) cloned in the same vector was used as control.
Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development
Article Snippet: .. Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 . .. A 29-mer non-effective oligonucleotide (Origene) cloned in the same vector was used as control.
Clone Assay:Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development
Article Snippet: .. Plasmid Constructs Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 . .. A 29-mer non-effective oligonucleotide (Origene) cloned in the same vector was used as control.
Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development
Article Snippet: .. Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 . .. A 29-mer non-effective oligonucleotide (Origene) cloned in the same vector was used as control.
Control:Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development
Article Snippet: .. Plasmid Constructs Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 . .. A 29-mer non-effective oligonucleotide (Origene) cloned in the same vector was used as control.
Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development
Article Snippet: .. Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 . .. A 29-mer non-effective oligonucleotide (Origene) cloned in the same vector was used as control.
Sequencing:Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development
Article Snippet: .. Plasmid Constructs Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 . .. A 29-mer non-effective oligonucleotide (Origene) cloned in the same vector was used as control.
Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development
Article Snippet: .. Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 . .. A 29-mer non-effective oligonucleotide (Origene) cloned in the same vector was used as control.
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